TITLE: ENZYME ELECTRICAL SENSOR ELECTRODE AND METHOD OF MAKING IT
European Patent Application EP0457892 A4

ABSTRACT:
Abstract not available for EP0457892
Abstract of corresponding document: US5082550
An electrochemical sensor electrode is formed from an electronic
conductor coated with a casting solution containing a perfluorosulfonic
acid ionomer and a selected enzyme. The selected enzyme catalyzes a
reaction between a predetermined substance in a solution and oxygen to
form an electrochemically active compound that is detected at the
electronic conductor. The resulting perfluorosulfonic acid polymer
provides a stable matrix for the enzyme for long lived enzyme activity,
wherein only thin coatings are required on the metal conductor. The
polymer also advantageously repels interfering substances from
contacting the enzyme and contains quantities of oxygen to maintain a
sensing capability during conditions of oxygen depletion in the sample.
In one particular embodiment, glucose oxidase is mixed with the
perfluorosulfonic acid ionomer to form an electrode for glucose
detection.

INVENTORS:
Rishpon, Judith
Gottesfeld, Shimson
Zawodzinski, Thomas A.

APPLICATION NUMBER: EP19910901386
PUBLICATION DATE: 10/28/1992
FILING DATE: 12/10/1990

ASSIGNEE: UNITED STATES DEPARTMENT OF ENERGY
INTERNATIONAL CLASSES: G01N27/327; C12Q1/00; (IPC1-7): G01N27/414
EUROPEAN CLASSES: C12Q1/00B2